{"id":3921,"date":"2021-05-28T15:14:31","date_gmt":"2021-05-28T15:14:31","guid":{"rendered":"https:\/\/enorcerna.com\/wiki\/definition\/recombinant-dna\/"},"modified":"2021-05-28T15:14:31","modified_gmt":"2021-05-28T15:14:31","slug":"recombinant-dna","status":"publish","type":"post","link":"https:\/\/enorcerna.com\/wiki\/definition\/recombinant-dna\/","title":{"rendered":"Recombinant DNA"},"content":{"rendered":"<div>\n<p>It is called <strong>DNA<\/strong> to the <strong>deoxyribonucleic acid<\/strong>, a biopolymer that makes up the genetic material that cells harbor.  DNA has the genetic information that living beings use to function and allows that information to be transmitted through heredity.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" loading=\"lazy\" src=\"https:\/\/enorcerna.com\/wiki\/wp-content\/uploads\/2021\/05\/adnrecombinante.jpg\" alt=\"Recombinant DNA\" width=\"243\" height=\"131\" class=\"alignright size-full wp-image-37102\" align=\"right\"\/>DNA is made up of a sequence of simple units, called <strong>nucleotides<\/strong> (which, in turn, are made up of a phosphate group, a nitrogenous base and a sugar).  When a <strong>molecule<\/strong> DNA is artificially formed from the union of different DNA sequences that come from two different organisms, we speak of <strong>Recombinant DNA<\/strong>.<\/p>\n<p>It can be said that recombinant DNA represents a <strong>artificial DNA molecule<\/strong>.  When said molecule created in vitro is introduced into a <strong>organism<\/strong>, a genetic alteration takes place that modifies its traits.<\/p>\n<p>The arrival of the creation and development of recombinant DNA, we have to emphasize that they occurred after researchers carried out numerous studies on the knowledge of restriction enzymes, the replication of viruses and plasmids, the replication and repair of DNA or the chemical synthesis of what are called nucleotide sequences.<\/p>\n<p>Specifically, it must be stated that it is considered that a key factor in the aforementioned DNA was none other than the set of projects carried out by Hamilton O. Smith and Daniel Nathans in the 1970s. More specifically, we refer to the They rushed to discover endonuclease proteins, which are also known by the name of restriction enzymes.<\/p>\n<p>To all the above we can add that it is very necessary to know that the recombinant DNA technique is currently used to a great extent both in the development of what are transgenic organisms and in the regulation of the production of protein synthesis, such as the case of insulin.  The process itself of the production of the aforementioned DNA we can determine that it is composed of six clearly delimited phases:<br \/>-The preparation of the DNA sequence to be able to undertake the pertinent cloning.<br \/>-The subsequent preparation of the cloning vector to be used.<br \/>-The formation itself of the recombinant DNA.<br \/>-The introduction of that in the host cell.<br \/>-The propagation of the crop.<br \/>-What would be the detection and selection of recombinant clones. <\/p>\n<p>For the development of recombinant DNA, biologists first work with a DNA molecule of a <strong>bacterium<\/strong>, a <strong>virus<\/strong>, a <strong>plant<\/strong> or another <strong>organism<\/strong>, manipulating it in the laboratory.  Then they introduce that molecule into a different organism.  This practice can be useful for creating vaccines or treating certain diseases.<\/p>\n<p>The generation of recombinant DNA involves the <strong>propagation of a DNA sequence<\/strong> that harbors a particular interest, to take it to an organism that does not have that sequence and, therefore, neither of its <strong>products<\/strong>.  From these processes, it is possible to obtain genetically altered microorganisms to develop drugs, obtain transgenic foods and create plants that resist the attack of pests.<\/p>\n<\/div>\n\n\n<div class=\"kk-star-ratings kksr-auto kksr-align-center kksr-valign-bottom\"\n    data-payload='{&quot;align&quot;:&quot;center&quot;,&quot;id&quot;:&quot;3921&quot;,&quot;slug&quot;:&quot;default&quot;,&quot;valign&quot;:&quot;bottom&quot;,&quot;ignore&quot;:&quot;&quot;,&quot;reference&quot;:&quot;auto&quot;,&quot;class&quot;:&quot;&quot;,&quot;count&quot;:&quot;0&quot;,&quot;legendonly&quot;:&quot;&quot;,&quot;readonly&quot;:&quot;&quot;,&quot;score&quot;:&quot;0&quot;,&quot;starsonly&quot;:&quot;&quot;,&quot;best&quot;:&quot;5&quot;,&quot;gap&quot;:&quot;4&quot;,&quot;greet&quot;:&quot;Rate this post&quot;,&quot;legend&quot;:&quot;0\\\/5 - (0 votes)&quot;,&quot;size&quot;:&quot;24&quot;,&quot;title&quot;:&quot;Recombinant DNA&quot;,&quot;width&quot;:&quot;0&quot;,&quot;_legend&quot;:&quot;{score}\\\/{best} - ({count} {votes})&quot;,&quot;font_factor&quot;:&quot;1.25&quot;}'>\n            \n<div class=\"kksr-stars\">\n    \n<div class=\"kksr-stars-inactive\">\n            <div class=\"kksr-star\" data-star=\"1\" style=\"padding-right: 4px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" data-star=\"2\" style=\"padding-right: 4px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" data-star=\"3\" style=\"padding-right: 4px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" data-star=\"4\" style=\"padding-right: 4px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" data-star=\"5\" style=\"padding-right: 4px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n    <\/div>\n    \n<div class=\"kksr-stars-active\" style=\"width: 0px;\">\n            <div class=\"kksr-star\" style=\"padding-right: 4px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" style=\"padding-right: 4px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" style=\"padding-right: 4px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" style=\"padding-right: 4px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" style=\"padding-right: 4px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 24px; height: 24px;\"><\/div>\n        <\/div>\n    <\/div>\n<\/div>\n                \n\n<div class=\"kksr-legend\" style=\"font-size: 19.2px;\">\n            <span class=\"kksr-muted\">Rate this post<\/span>\n    <\/div>\n    <\/div>\n","protected":false},"excerpt":{"rendered":"<p>It is called DNA to the deoxyribonucleic acid, a biopolymer that makes&hellip;<\/p>\n","protected":false},"author":1,"featured_media":3922,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-3921","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-definition"],"_links":{"self":[{"href":"https:\/\/enorcerna.com\/wiki\/wp-json\/wp\/v2\/posts\/3921","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/enorcerna.com\/wiki\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/enorcerna.com\/wiki\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/enorcerna.com\/wiki\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/enorcerna.com\/wiki\/wp-json\/wp\/v2\/comments?post=3921"}],"version-history":[{"count":0,"href":"https:\/\/enorcerna.com\/wiki\/wp-json\/wp\/v2\/posts\/3921\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/enorcerna.com\/wiki\/wp-json\/wp\/v2\/media\/3922"}],"wp:attachment":[{"href":"https:\/\/enorcerna.com\/wiki\/wp-json\/wp\/v2\/media?parent=3921"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/enorcerna.com\/wiki\/wp-json\/wp\/v2\/categories?post=3921"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/enorcerna.com\/wiki\/wp-json\/wp\/v2\/tags?post=3921"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}